出版社：American Society for Biochemistry and Molecular Biology
摘要：The source for triacylglycerol-rich lipoprotein cholesterol was investigated in CaCo-2 cells grown on filters separating an upper and a lower well. Oleic acid, a fatty acid that promotes triacylglycerol-rich lipoprotein synthesis and secretion in CaCo-2 cells, increased the vesicular-mediated influx of plasma membrane cholesterol to the endoplasmic reticulum. Unesterified and esterified cholesterol derived from the plasma membrane were increased in triacylglycerol-rich lipoproteins secreted by cells incubated with oleic acid. Fatty acids, which increased the number of lipoprotein particles secreted (increased apoB secretion), increased plasma membrane cholesterol influx and secretion. Oleic acid caused a modest increase in the synthesis of cholesterol and a two-fold increase in cholesteryl esters. The amount of newly synthesized cholesterol secreted in lipoproteins of density
