出版社：American Society for Biochemistry and Molecular Biology
摘要：The skin sterols of normal rats and of rats treated with the drug triparanol have been analyzed by means of chromatography on silicic acid followed by gas-liquid chromatography of the components of the peaks obtained from the silicic acid chromatogram. These two procedures complement each other in leading to complete separations of pairs of sterols that are poorly separated in either system alone. The results are shown to indicate the presence in rat skin of several previously undetected compounds for which molecular structures are proposed on the basis of the retention factor method. Evidence is presented for at least two effects of the drug triparanol, (1) in causing accumulation of the Δ24-analogues of all the intermediates in cholesterol biosyntheis that normally occur in the 24,25-dihydro form, and (2) in causing marked alterations in the proportions of Δ7- to Δ8-isomers of these intermediates. The significance of these findings is discussed in the light of current views of the pathway of cholesterol biosynthesis.
