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  • 标题:Purification and partial characterization of sphingomyelin from human plasma
  • 本地全文:下载
  • 作者:Charles C. Sweeley
  • 期刊名称:JLR Papers In Press
  • 印刷版ISSN:0022-2275
  • 电子版ISSN:1539-7262
  • 出版年度:1963
  • 卷号:4
  • 期号:4
  • 页码:402-406
  • 语种:English
  • 出版社:American Society for Biochemistry and Molecular Biology
  • 摘要:Isolation of pure sphingomyelin from human plasma on a preparative scale is described. Analyses of fatty acids by gas-liquid chromatography on two polar polyester columns indicate the presence of all even- and odd-carbon, saturated fatty acids from C14 to C25 and most of the corresponding monoenoic acids as well. Palmitic acid is the predominant component; seven of the acids constitute over 90% of the total fraction. A previously unassigned trace constituent of the fatty acid fraction has been tentatively identified as a C24 dienoic acid on the basis of relative retention times, microhydrogenation, and reaction with mercuric acetate.
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