出版社：American Society for Biochemistry and Molecular Biology
摘要：The St. Thomas' mixed hyperlipidemic (SMHL) rabbit (previously St. Thomas' Hospital rabbit) is a putative model of familial combined hyperlipidemia (FCH). When fed a low (0.08%) cholesterol diet, it exhibits elevations in both plasma cholesterol and triglyceride compared to New Zealand White (NZW) controls. To determine the mechanism for this hyperlipidemia we studied the secretion of apolipoprotein B (apoB)-containing lipoproteins from perfused livers of both young and mature rabbits. During a 3-h perfusion we measured the total cholesterol and triglyceride content of the medium and the cholesterol, triglyceride, and apoB content of very low density lipoprotein (VLDL)1 (Sf 60–400), VLDL2 (Sf 20–60), intermediate (Sf 12–20), and low (Sf 0–12) density lipoproteins (IDL, LDL). Lipoprotein concentrations increased linearly throughout the perfusion period. The rate of cholesterol output was 3-fold higher (459 vs. 137 ng/g liver/min, P = 0.003) in SMHL versus NZW rabbits whilst that of triglyceride was similar (841 vs. 662 ng/g liver/min, NS). VLDL1 cholesterol output was elevated 2-fold (232 vs. 123 ng/g liver/min, P 2 + IDL + LDL cholesterol output, 4.5-fold (106 vs. 23 ng/g liver/min, P 2 + IDL + LDL apoB was increased 9-fold at 53 versus 6 ng/g liver per min in NZW ( P We conclude that the SMHL rabbit overproduces apoB-containing lipoproteins particularly in the VLDL2 + IDL + LDL fraction, a characteristic consistent with its use as a model of FCH.— Ardern, H. A., G. M. Benson, K. E. Suckling, M. J. Caslake, J. Shepherd, and C. J. Packard. Apolipoprotein B overproduction by the perfused liver of the St. Thomas' mixed hyperlipidemic (SMHL) rabbit. J. Lipid Res. 1999. 40: 2234–2243.
关键词：liver perfusion ; lipoprotein production ; VLDL ; IDL ; LDL ; apoB production ; Froxfield Mixed hyperlipidemic rabbit ; St. Thomas Hospital rabbit
