出版社：American Society for Biochemistry and Molecular Biology
摘要：Percent cholesterol absorption was measured in 94 normal subjects aged 17–80 years while consuming diets generally low in cholesterol (mean intake = 226 ± 126 mg/day). A new dual stable isotope method was used where a cholesterol tracer containing 6 extra mass units was given intravenously and another tracer with 5 extra mass units was given orally during a standard test meal. The ratio of tracers in plasma was determined by negative ion mass spectrometry of pentafluorobenzoyl sterol esters. Absorption values ranged widely from 29.0% to 80.1% with mean 56.2 ± 12.1 (SD) %. Cholesterol absorption was significantly increased in African-Americans (63.4 ± 11.8% vs. 55.1 ± 11.9%, P = 0.027) but was similar for women (53.3 ± 11.9%) and men (57.6 ± 12.1%). It was not related to plasma lipoproteins, age, apoE3/E3 or E3/E4 genotype, or chronic dietary intake of energy, fat, or cholesterol quantitated from 7-day food records. However, dietary cholesterol intake was positively related to plasma cholesterol ( P = 0.036) and triglycerides ( P = 0.026). The milligram amount of dietary cholesterol absorbed (but not percent absorption) was positively correlated with fasting plasma insulin ( r = 0.525, P r = 0.367, P = 0.0003) and glucagon ( r = 0.421, P —Bosner, M. S., L. G. Lange, W. F. Stenson, and R. E. Ostlund, Jr. Percent cholesterol absorption in normal women and men quantified with dual stable isotopic tracers and negative ion mass spectrometry. J. Lipid Res. 1999. 40: 302–308.
