出版社：American Society for Biochemistry and Molecular Biology
摘要：Estrogen replacement therapy has been shown to attenuate atherogenesis, although the mechanisms for this effect are incompletely defined. Previously, we showed that 17-β estradiol (estradiol) attenuated oxidant stress-induced increases in vascular low density lipoprotein (LDL) accumulation. It was unclear whether estradiol's effect was imparted on the lipoprotein particle or the artery wall. To examine this, we chronically treated rats with the following sex hormones: low estradiol, high estradiol, progesterone, low estradiol + progesterone, placebo, or control. Carotid arteries (n = 8/group) were isolated and perfused with fluorescently labeled LDL. Rates of LDL accumulation were measured before and after treatment with 10 ng/ml tumor necrosis factor-α (TNF) using quantitative fluorescence microscopy. We observed a 50% decrease in basal LDL accumulation rates ( P P P = 0.451), while incubation of LDL with 65 pg/ml estradiol attenuated the TNF effect ( P 1 ) decreased endothelial layer permeability; and 2 ) incorporation of estradiol into the LDL particle and prevention of LDL binding to the artery wall. — Walsh, B. A., A. E. Mullick, C. E. Banka, and J. C. Rutledge. 17β-Estradiol acts separately on the LDL particle and artery wall to reduce LDL accumulation. J. Lipid Res. 2000. 41: 134–141.
