出版社：American Society for Biochemistry and Molecular Biology
摘要：N -acyl-ethanolamine phospholipids (NAPE) can be formed as a stress response during neuronal injury, and they are precursors for N -acyl-ethanolamines (NAE), some of which are endocannabinoids. The levels of NAPE accumulated during post-decapitative ischemia (6 h at 37°C) were studied in rat brains of various age (1, 6, 12, 19, 30, and ~70 days) by the use of 31P NMR spectroscopy of lipid extracts. This ability to accumulate NAPE was compared with the activity of N -acyltransferase and of NAPE-hydrolyzing phospholipase D (NAPE-PLD) in brain microsomes. These two enzymes are involved in the formation and degradation of NAPE, respectively. The results showed that 1 ) the ability to accumulate NAPE during post-decapitative ischemia is especially high in the youngest rats and is markedly reduced in older brains [in 1-day-old rat brains NAPE accumulated to 1.5% of total phospholipids, while in 30-day-old rat brains NAPE accumulation could not be detected (detection limit 0.09 %)]; and 2 ) this age pattern of accumulation can be explained by a combination of the decreased activity of N -acyltransferase and the increased activity of NAPE-PLD during development. These results point out that it would be advantageous to investigate a potential cytoprotective role of NAPE in the brains of very young rats. —Moesgaard, B., G. Petersen, J. W. Jaroszewski, and H. S. Hansen. Age dependent accumulation of N -acyl-ethanolamine phospholipids in ischemic rat brain: a 31P NMR and enzyme activity study. J. Lipid Res. 41: 985–990.
关键词：anandamide ; ischemia ; rat brain development ; N -acyltransferase ; N -acyl-ethanolamine phospholipid-hydrolyzing phospholipase D ; 31P NMR.
