出版社：American Society for Biochemistry and Molecular Biology
摘要：CD11b is an α chain of the leukocyte β2-integrin, Mac-1, which mediates binding and extravasation of leukocytes. Because this event is critical in atherosclerosis, we examined the role of CD11b in lesion formation. Atherosclerosis-susceptible, low density lipoprotein receptor-deficient (LDL-R−/−) mice were irradiated and repopulated with bone marrow cells from CD11b-deficient (CD11b−/−) mice. After 4 weeks, −/− bone marrow-transplanted LDL-R−/− mice expressed CD11b, whereas ~25% of the CD11b+/+ bone marrow-transplanted LDL-R−/− mice expressed CD11b. After consuming a high-fat diet for 16 weeks the mean lesion aortic valve area, cholesterol accumulation in the aorta, and the degree of intimal macrophage infiltration were similar in mice reconstituted with either CD11b+/+ or CD11b−/− bone marrow cells. The studies confirm that CD11b expression of bone marrow-derived cells does not influence the development of atherosclerosis in hypercholesterolemic LDL-R−/− mice. —Kubo, N., W. A. Boisvert, C. M. Ballantyne, and L. K. Curtiss. Leukocyte CD11b expression is not essential for the development of atherosclerosis in mice. J. Lipid Res. 2000. 41: 1060–1066.
关键词：transgenic/knockout ; fluorescence-activated cell sorting ; in vivo animal models ; adhesion molecules ; inflammation
