出版社：American Society for Biochemistry and Molecular Biology
摘要：Serum paraoxonase (PON) is associated with plasma high density lipoproteins, and prevents the oxidative modification of low density lipoproteins. We have developed a sensitive sandwich enzyme-linked immunosorbent assay (ELISA), using two monoclonal antibodies against PON, to measure serum PON concentration. The concentration of PON in healthy Japanese subjects was 59.3 ± 1.3 μg/mL (mean ± SEM; n = 87). Serum PON concentrations in relation to the PON 192 genetic polymorphism were: 69.5 ± 2.9 μg/mL in the QQ genotype; 63.0 ± 1.9 μg/mL in the QR genotype; and 52.8 ± 1.7 μg/mL in the RR genotype. Concentrations were significantly lower in the RR than in the QQ genotype ( P P P P —Kujiraoka, T., T. Oka, M. Ishihara, T. Egashira, T. Fujioka, E. Saito, S. Saito, N. E. Miller, and H. Hattori. A sandwich enzyme-linked immunosorbent assay for human serum paraoxonase concentration. J. Lipid Res. 2000. 41: 1358–1363.
关键词：low density lipoproteins ; high density lipoproteins ; phospholipids ; coronary heart disease
