出版社：American Society for Biochemistry and Molecular Biology
摘要：It has been shown that estrogens need to be metabolized to their hydrophobic estrogen ester derivatives to act as antioxidants in lipoproteins. Data suggest that 17β-estradiol (E2) becomes esterified in LCAT-induced reactions and the esters are transported from HDL particles to LDL and VLDL particles by a CETP-dependent mechanism. In the present study we have further investigated the regulation of E2 esterification by LCAT and focused on the importance of HDL structure and composition in the esterification process. Isolated LDL, HDL2, HDL3, and reconstituted discoidal HDL (rHDL) were incubated with labeled E2, with and without purified LCAT, at 37°C for 24 h. After purification of the lipoprotein fractions, there was a significant peak of radioactivity representing esterified estradiol attached to HDL3 and rHDL, but HDL2 and LDL contained only trace amounts of labeled estradiol ester. TLC analysis confirmed that the radioactivity migrated in a position corresponding to that of 17β-E2 17-monoester standard. The amount of radioactivity associated with HDL3 and rHDL representing esterified E2 was significantly increased by addition of purified LCAT. However, only limited increases of radioactivity were observed in HDL2 and LDL. In conclusion, HDL subfractions differ in their potential to regulate estradiol esterification by LCAT. —Höckerstedt, A., M. J. Tikkanen, and M. Jauhiainen. LCAT facilitates transacylation of 17β-estradiol in the presence of HDL3 subfraction. J. Lipid Res. 2002. 43: 392–397.
关键词：estrogen ; estradiol ester ; lecithin:cholesterol acyltransferase ; high density lipoprotein 2 ; high density lipoprotein 3 ; reconstituted discoidal HDL
