出版社：American Society for Biochemistry and Molecular Biology
摘要：Apolipoprotein (apo)A-I, the major protein component of HDL, is synthesized principally in the small intestine and liver. Recently we observed an increase in plasma apoA-I level in humans who were on an oxidized fat diet. To test whether oxidized fatty acids could affect apoA-I synthesis, we incubated day 4 (undifferentiated) and day 14 (differentiated) Caco-2 cells with varying concentrations of oxidized linoleic acid (ox-linoleic acid) (5, 10, and 25 μM) and unoxidized linoleic acid for 24 h. Ox-linoleic acid caused a dose-dependent increase in the levels of apoA-I protein in both differentiated and undifferentiated Caco-2 cells as assessed by ELISA and Western blot analysis. Whereas apoB production was not increased by ox-linoleic acid in both day 4 and day 14 Caco-2 cells. The mRNA expression for apoA-I paralleled the protein expression when measured by RT-PCR. We also found that both day 4 and day 14 Caco-2 cells did express peroxisomal proliferator-activated receptor-γ (PPAR-γ). mRNA and PPAR-γ ligand could increase apoA-I secretion in these cells. Therefore we propose that the mechanism for the induction of apoA-I might include PPARγ for which oxidized fatty acid is a ligand. —Rong, R., S. Ramachandran, M. Penumetcha, N. Khan, and S. Parthasarathy. Dietary oxidized fatty acids may enhance intestinal apolipoprotein A-I production. J. Lipid Res. 2002. 43: 557–564.
关键词：atherosclerosis ; oxidized linoleic acid ; Caco-2 cells ; high density lipoprotein ; oxidative stress ; brush border ; 13-HPODE ; 13-HODE ; antioxidant defense ; oxidized low density lipoprotein ; apolipoprotein B ; PPAR-γ
