出版社：American Society for Biochemistry and Molecular Biology
摘要：Lysophosphatidic acid (LPA) is a lipid mediator that may play an important role in wound healing, embryonic development, and progression of cancer. Here, we report a procedure for the quantification of LPA by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The method is based on a characteristic mass shift with total charge change (from –2 to +1) of the phosphate species due to 1:1 complexation of LPA2− with a dinuclear zinc (II) complex {1,3-bis[bis(pyridin-2-ylmethyl)amino]propan-2-olato dizinc(II) complex; Zn2L3+} at physiological pH. The monocationic complex [LPA2−-Zn2L3+]+ was detected in the positive mode, in which no other signal of cation adducts of LPA2− was observed. The detection limit of 18:1 LPA by this method was 0.1 pmol on a sample plate. The intensity ratio of [LPA2−-Zn2L3+]+ against an internal standard [17:0 LPA2−-Zn2L3+]+ increased linearly with their molar ratio. Based on the relative intensities of complex ions, we determined the amounts of LPA homologs in an egg white by this method; the results obtained were in good agreement with those by gas liquid chromatography. This sensitive and convenient procedure for LPA-specific detection is useful for the quantification of LPA homologs occurring in biological materials.
