出版社：American Society for Biochemistry and Molecular Biology
摘要：A mosquito sterol carrier protein-2, AeSCP-2, has been shown to aid in the uptake of cholesterol in mosquito cells. The discovery of chemical inhibitors of AeSCP-2 is reported here. AeSCP-2 inhibitors (SCPIs) belong to several chemotypes of hydrophobic compounds. Those inhibitors competed with cholesterol for AeSCP-2, binding with relatively high binding affinities. In cultured insect cells, SCPIs reduced cholesterol uptake by as much as 30% at 1–5 μM concentrations. SCPIs were potent larvicides to the yellow fever mosquito, Aedes aegypti , and to the tobacco hornworm, Manduca sexta , with 50% lethal doses (LD50s) of 5–21 μM and 0.013–15 ng/mg diet, respectively. The results indicate that sterol carrier protein-2 has functional similarity in two different insect species.
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