出版社：American Society for Biochemistry and Molecular Biology
摘要：The ability of human postprandial triacylglycerol-rich lipoproteins (TRLs), isolated after meals enriched in saturated fatty acids (SFAs), n-6 PUFAs, and MUFAs, to inhibit the uptake of 125I-labeled LDL by the LDL receptor was investigated in HepG2 cells. Addition of TRLs resulted in a dose-dependent inhibition of heparin-releasable binding, cell-associated radioactivity, and degradation products of 125I-labeled LDL ( P f) 60–400 resulted in significantly greater inhibition of cell-associated radioactivity than PUFA-rich particles ( P = 0.016) and total uptake of 125I-labeled LDL compared with PUFA- and MUFA-rich particles ( P 125I-labeled LDL. Real time RT-PCR showed that the SFA-rich Sf 60–400 increased the expression of genes involved in hepatic lipid synthesis ( P P = 0.008). In conclusion, these findings suggest an alternative or additional mechanism whereby acute fat ingestion can influence LDL clearance via competitive apoE-dependent effects of TRL on the LDL receptor.
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