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  • 标题:Perinatal n-3 fatty acid deficiency selectively reduces myo-inositol levels in the adult rat PFC: an in vivo 1H-MRS study
  • 本地全文:下载
  • 作者:Robert K. McNamara ; Jessica Able ; Ronald Jandacek
  • 期刊名称:JLR Papers In Press
  • 印刷版ISSN:0022-2275
  • 电子版ISSN:1539-7262
  • 出版年度:2009
  • 卷号:50
  • 期号:3
  • 页码:405-411
  • DOI:10.1194/jlr.M800382-JLR200
  • 语种:English
  • 出版社:American Society for Biochemistry and Molecular Biology
  • 摘要:To investigate the effects of omega-3 fatty acid deficiency on phosphatidylinositol signaling in brain, myo -inositol (mI) concentrations were determined in the prefrontal cortex (PFC) of omega-3 fatty acid deficient rats by in vivo proton magnetic resonance spectroscopy (1H-MRS). To generate graded deficits in PFC docosahexaenoic acid (22:6 n -3) (DHA) composition, perinatal and postweaning α-linolenic acid (18:3 n -3) (ALA) deficiency models were used. Adult male rats were scanned in a 7T Bruker Biospec system and a 1H-MRS spectrum acquired from the bilateral medial PFC. Rats were then challenged with SKF83959, a selective agonist at phosphoinositide (PI)-coupled dopamine D1 receptors. Postmortem PFC fatty acid composition was determined by gas chromatography. Relative to controls, PFC DHA composition was significantly reduced in adult postweaning (−27%) and perinatal (−65%) ALA-deficiency groups. Basal PFC mI concentrations were significantly reduced in the perinatal deficiency group (−21%, P = 0.001), but not in the postweaning deficiency group (−1%, P = 0.86). Among all rats, DHA composition was positively correlated with mI concentrations and the mI/creatine (Cr) ratio. SKF83959 challenge significantly increased mI concentrations only in the perinatal deficiency group (+16%, P = 0.02). These data demonstrate that perinatal deficits in cortical DHA accrual significantly and selectively reduce mI concentrations and augment receptor-generated mI synthesis.
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