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  • 标题:Comprehensive quantification of ceramide species in human stratum corneum
  • 本地全文:下载
  • 作者:Yoshinori Masukawa ; Hirofumi Narita ; Hirayuki Sato
  • 期刊名称:JLR Papers In Press
  • 印刷版ISSN:0022-2275
  • 电子版ISSN:1539-7262
  • 出版年度:2009
  • 卷号:50
  • 期号:8
  • 页码:1708-1719
  • DOI:10.1194/jlr.D800055-JLR200
  • 语种:English
  • 出版社:American Society for Biochemistry and Molecular Biology
  • 摘要:One of the key challenges in lipidomics is to quantify lipidomes of interest, as it is practically impossible to collect all authentic materials covering the targeted lipidomes. For diverse ceramides (CER) in human stratum corneum (SC) that play important physicochemical roles in the skin, we developed a novel method for quantification of the overall CER species by improving our previously reported profiling technique using normal-phase liquid chromatography-electrospray ionization-mass spectrometry (NPLC-ESI-MS). The use of simultaneous selected ion monitoring measurement of as many as 182 kinds of molecular-related ions enables the highly sensitive detection of the overall CER species, as they can be analyzed in only one SC-stripped tape as small as 5 mm × 10 mm. To comprehensively quantify CERs, including those not available as authentic species, we designed a procedure to estimate their levels using relative responses of representative authentic species covering the species targeted, considering the systematic error based on intra-/inter-day analyses. The CER levels obtained by this method were comparable to those determined by conventional thin-layer chromatography (TLC), which guarantees the validity of this method. This method opens lipidomics approaches for CERs in the SC.—Masukawa, Y., H. Narita, H. Sato, A. Naoe, N. Kondo, Y. Sugai, T. Oba, R. Homma, J. Ishikawa, Y. Takagi, and T. Kitahara. Comprehensive quantification of ceramide species in human stratum corneum. J. Lipid. Res. 2009. 50: 1708–1719.
  • 关键词:high sensitivity ; normal-phase liquid chromatography–electrospray ionization-mass spectrometry ; relative response ; simultaneous selected ion monitoring ; skin
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