出版社：American Society for Biochemistry and Molecular Biology
摘要：A simple, high-yielding preparation of monolysocardiolipin (MLCL) by phospholipase A2 hydrolysis of cardiolipin (CL) in methanol on a semi-preparative scale is described. In methanol, phospholipase A2 preferentially hydrolyzes CL to MLCL. This selectivity results in ∼80% yield of MLCL. The synthesized MLCL and dilysocardiolipin were characterized by NMR and ESI-MS/MS. Only the sn -2 position of CL was hydrolyzed by phospholipase A2 in methanol.
关键词：cardiolipin ; phospholipase ; mass spectrometry ; dilysocardiolipin
