首页    期刊浏览 2024年11月07日 星期四
登录注册

文章基本信息

  • 标题:Measurement of serum PON-3 concentration: method evaluation, reference values, and influence of genotypes in a population-based study
  • 本地全文:下载
  • 作者:Gerard Aragonès ; Marta Guardiola ; María Barreda
  • 期刊名称:JLR Papers In Press
  • 印刷版ISSN:0022-2275
  • 电子版ISSN:1539-7262
  • 出版年度:2011
  • 卷号:52
  • 期号:5
  • 页码:1055-1061
  • DOI:10.1194/jlr.D014134
  • 语种:English
  • 出版社:American Society for Biochemistry and Molecular Biology
  • 摘要:Experimental studies showed that paraoxonase-3 (PON3) retards lipoprotein oxidation. Our objective was to describe a new assay to measure serum PON3 concentrations and report their reference values in a population-based study. The influence of PON3 promoter polymorphisms and their relationships with PON1 and lipid profile were also studied. We generated an anti-PON3 antibody by inoculating rabbits with a synthetic peptide specific to mature PON3. This antibody was used to develop an ELISA. The average regression line of standard plots (n = 8) was y = 0.9587 (0.3392) log10 x + 1.9466 (0.0861) [ r 2 = 0.924 (0.0131); P
  • 关键词:analytical methods ; antioxidants ; atherosclerosis ; lipoproteins ; oxidative stress
国家哲学社会科学文献中心版权所有