出版社：American Society for Biochemistry and Molecular Biology
摘要：Wax esters are neutral lipids exhibiting desirable properties for lubrication. Natural sources have traditionally been whales. Additionally some plants produce wax esters in their seed oil. Currently there is no biological source available for long chain length monounsaturated wax esters that are most suited for industrial applications. This study aimed to identify enzymatic requirements enabling their production in oilseed plants. Wax esters are generated by the action of fatty acyl-CoA reductase (FAR), generating fatty alcohols and wax synthases (WS) that esterify fatty alcohols and acyl-CoAs to wax esters. Based on their substrate preference, a FAR and a WS from Mus musculus were selected for this study ( Mm FAR1 and Mm WS). Mm WS resides in the endoplasmic reticulum (ER), whereas Mm FAR1 associates with peroxisomes. The elimination of a targeting signal and the fusion to an oil body protein yielded variants of Mm FAR1 and Mm WS that were cotargeted and enabled wax ester production when coexpressed in yeast or Arabidopsis . In the fae1 fad2 double mutant, rich in oleate, the cotargeted variants of Mm FAR1 and Mm WS enabled formation of wax esters containing >65% oleyl-oleate. The data suggest that cotargeting of unusual biosynthetic enzymes can result in functional interplay of heterologous partners in transgenic plants.
