出版社：American Society for Biochemistry and Molecular Biology
摘要：The interaction of dietary fats and carbohydrates on liver mitochondria were examined in male FBNF1 rats fed 20 different low-fat isocaloric diets. Animal growth rates and mitochondrial respiratory parameters were essentially unaffected, but mass spectrometry-based mitochondrial lipidomics profiling revealed increased levels of cardiolipins (CLs), a family of phospholipids essential for mitochondrial structure and function, in rats fed saturated or trans fat-based diets with a high glycemic index. These mitochondria showed elevated monolysocardiolipins (a CL precursor/product of CL degradation), elevated ratio of trans -phosphocholine (PC) (18:1/18:1) to cis -PC (18:1/18:1) (a marker of thiyl radical stress), and decreased ubiquinone Q9 ; the latter two of which imply a low-grade mitochondrial redox abnormality. Extended analysis demonstrated: i ) dietary fats and, to a lesser extent, carbohydrates induce changes in the relative abundance of specific CL species; ii ) fatty acid (FA) incorporation into mature CLs undergoes both positive (>400-fold) and negative (2.5-fold) regulation; and iii ) dietary lipid abundance and incorporation of FAs into both the CL pool and specific mature tetra-acyl CLs are inversely related, suggesting previously unobserved compensatory regulation. This study reveals previously unobserved complexity/regulation of the central lipid in mitochondrial metabolism.
