出版社：American Society for Biochemistry and Molecular Biology
摘要：Lysophospholipids (LysoGPs) serve as lipid mediators and precursors for synthesis of diacyl phospholipids (GPs). LysoGPs detected in cells have various acyl chains attached at either the sn -1 or sn -2 position of the glycerol backbone. In general, acyl chains at the sn -2 position of 2-acyl-1-LysoGPs readily move to the sn -1 position, generating 1-acyl-2-lyso isomers by a nonenzymatic reaction called intra-molecular acyl migration, which has hampered the detection of 2-acyl-1-LysoGPs in biological samples. In this study, we developed a simple and versatile method to separate and quantify 2-acyl-1- and 1-acyl-2-LysoGPs. The main point of the method was to extract LysoGPs at pH 4 and 4°C, conditions that were found to completely eliminate the intra-molecular acyl migration. Under the present conditions, the relative amounts of 2-acyl-1-LysoGPs and 1-acyl-2-LysoGPs did not change at least for 1 week. Further, in LysoGPs extracted from cells and tissues under the present conditions, most of the saturated fatty acids (16:0 and 18:0) were found in the sn -1 position of LysoGPs, while most of the PUFAs (18:2, 20:4, 22:6) were found in the sn -2 position. Thus the method can be used to elucidate the in vivo role of 2-acyl-1-LysoGPs.
关键词：acyl migration reaction ; asymmetric distribution of fatty acid ; biological membrane
