出版社：American Society for Biochemistry and Molecular Biology
摘要：The in vitro conversion of dl -mevalonate-2-C14 and cholesterol-4-C14 to 5α-cholestan-3β-ol was studied in liver homogenates of rats and guinea pigs. On the average 0.01–0.05% of the substrate radioactivity was converted to 5α-cholestan-3β-ol by the livers of both species during the 4 hr incubation period. The biosynthesis of 5α-cholestan-3β-ol by cell-free liver homogenates in vitro demonstrates that the presence of this saturated sterol is at least partly due to biosynthesis by mammalian tissues, and cannot be ascribed exclusively to bacterial action upon cholesterol in the intestine.
