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  • 标题:Purity of cholesterol to be used as a primary standard
  • 本地全文:下载
  • 作者:J. H. Williams ; M. Kuchmak ; R. F. Witter
  • 期刊名称:JLR Papers In Press
  • 印刷版ISSN:0022-2275
  • 电子版ISSN:1539-7262
  • 出版年度:1965
  • 卷号:6
  • 期号:4
  • 页码:461-465
  • 语种:English
  • 出版社:American Society for Biochemistry and Molecular Biology
  • 摘要:Cholesterol preparations from 14 commercial sources were purified by the dibromide method. The cholesterol contents of the original and purified preparations were determined by gas–liquid chromatography. Calculation of the results of the gas chromatographic analyses from the areas of the peaks rather than from their heights gave more precise results. Purified samples had higher contents of cholesterol, higher melting points, and narrower melting point ranges, and exhibited fewer contaminants on thin-layer chromatoplates than did the original preparations.
  • 关键词:cholesterol ; primary standard ; purification ; cholesterol dibromide ; quantitative gas–liquid chromatography ; thin-layer chromatography
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