出版社：American Society for Biochemistry and Molecular Biology
摘要：Two-stage thin-layer chromatography on Aluminum Oxide G has been used to separate triglycerides, cholesterol, cholesterol esters, and diglycerides from contaminating fatty acids present in the lipid mixtures. Negligible hydrolysis of glycerol esters occurs. The method is particularly useful in metabolic experiments conducted in vitro, in which radioactive fatty acids are employed as precursors.
