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  • 标题:Use of constant composition polyvinylpyrrolidone columns to study the interaction of fat particles with plasma
  • 本地全文:下载
  • 作者:David D. O'Hara ; Daniel Porte ; Robert H. Williams
  • 期刊名称:JLR Papers In Press
  • 印刷版ISSN:0022-2275
  • 电子版ISSN:1539-7262
  • 出版年度:1966
  • 卷号:7
  • 期号:2
  • 页码:264-269
  • 语种:English
  • 出版社:American Society for Biochemistry and Molecular Biology
  • 摘要:Fat particles (lipoproteins of Sf > 400) have been obtained from dog and human lymph and from human post-alimentary plasma. They were flocculated by a solution of 5% polyvinylpyrrolidone (PVP) in 10% NaCl and layered at the bottom of tubes of PVP of various concentrations between 2% and 5% (w/v). As the concentration of PVP increased, a greater proportion of the particles accumulated at the top of the tube (``primary'' particles). When the concentration of PVP in the tube was held constant at 3% PVP, dilution of the sample with plasma was found to produce an increased proportion of bottom particles (``secondary'' particles). This observation suggests that bottom particles result from an interaction of top particles with plasma.
  • 关键词:dog ; man ; lymph ; plasma ; primary ; secondary ; fat particles ; interconversion ; separation ; polyvinylpyrrolidone column ; constant composition ; gradient ; very low density lipoproteins
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