出版社：American Society for Biochemistry and Molecular Biology
摘要：Lecithinase activity in post-heparin serum has been demonstrated. Phosphatidyl choline (PC) can be degraded to lysophosphatidyl choline and fatty acids at a rate of more than 1 μmole/hr per ml of serum in an incubation system containing PC, 0.1 m glycine-NaOH buffer (pH 9.6), and deoxycholate. This activity cannot be found in serum obtained prior to the injection of heparin. Post-heparin serum lecithinase can be distinguished from the heat-stable pancreatic lecithinase by the markedly different effects of heat, paraoxon, and EDTA, and from serum lecithin: cholesterol acyltransferase by the differential effect of p -hydroxymercuribenzoate. In contrast to the acyltransferase and to pancreatic lecithinase, which are active at the β (C-2) position of lecithin, post-heparin serum lecithinase is active at α′ (C-1) position.
