出版社：American Society for Biochemistry and Molecular Biology
摘要：This paper describes the trimethylsilylation of sphingolipid bases under conditions that give derivatives of improved stability. The retention times of the common C18 and C20 long-chain bases, including the anhydro bases, obtained on a commercially available gas-liquid chromatographic column with a nonpolar stationary phase are given. Data are also presented on the separation of the erythro and threo isomers of sphingosine and dihydrosphingosine, as the trimethylsilyl ethers of the N -acetyl derivatives.
关键词：sphingolipid bases ; gas-liquid chromatography ; trimethylsilyl ether derivatives ; separation ; long-chain bases ; erythro and threo isomers ; trimethylsilylation ; N -acetyl bases ; C18- and C20-sphingosine, dihydrosphingosine, and phytosphingosine ; dehydrophytosphingosine ; anhydro-C18-phytosphingosine ; anhydro-C18-dehydrophytosphingosine
