出版社：American Society for Biochemistry and Molecular Biology
摘要：1,2-3H-Cholesterol was fed to a subject together with 4-14C-cholesterol at a known 3H/14C ratio. The ratio was satisfactorily preserved in cholesterol recovered from plasma, red cells, and bile, and in bile acids of bile. Isotopic fractionation was seen during thin-layer chromatographic isolation of cholesterol. In work with 1,2-3H-cholesterol or its metabolites care should be taken to recover chromatographic bands in their entirety.
关键词：cholesterol ; 1,2-3H-cholesterol ; 4-14C-cholesterol ; thin-layer chromatography ; isotope effect ; plasma ; red blood cells ; bile acids ; man
