This study investigated the impact of Human Resource Management (HRM) practices on organizational commitment among pharmacists working in chain pharmacies in Jordan. The study was conducted across pharmacists of different levels in two chain pharmacies in Jordan. A questionnaire was designed and distributed to pharmacist working in two chain pharmacies in Jordan. Results found that training and development and reward system were positively and significantly impacted affective commitment; as well as both recruitment and selection and reward system were positively and significantly affected continuance commitment. However, no significant impact found between normative commitment and any of the suggested human recourse management practices. The results showed that the majority of the variables confirmed previous studies and the remaining minority did not support these studies due to factors such as culture, values, and the nature of health care practice. The results have a great implication for the pharmacy business sector in Jordan.