Considering that workers in healthcare institutions are most at risk of workplace stress (WPS), and that career commitment (CC) is a fundamental requirement for organizational success, this descriptive and quantitative survey study attempts to provide up-to-date details on the extent of WPS and CC and the impact of WPS on CC in Comprehensive Health Centers (CHCs) in Jordan during 2015 and 2016. Four hundred workers were randomly selected from twelve CHCs; the response rate was 73%. The study used the software SPSS version (15.0) for Windows to examine the data. The study produced a number of findings, with the results of mean and standard deviations revealing that the presence of WPS is high and CC is low among CHCs workers. Multiple regression tests showed that high levels of WPS have a negative impact on CC. The study results therefore suggest that appropriate interventions to control WPS may be useful to improve CC in CHCs in Jordan.