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  • 标题:Case of contamination by Listeria monocytogenes in mozzarella cheese
  • 作者:Sara Greco ; Rita Tolli ; Teresa Bossù
  • 期刊名称:Italian Journal of Food Safety
  • 电子版ISSN:2239-7132
  • 出版年度:2014
  • 卷号:3
  • 期号:1
  • DOI:10.4081/ijfs.2014.1708
  • 语种:English
  • 出版社:PAGEPress Publications
  • 摘要:Following a Listeria monocytogenes detection in a mozzarella cheese sampled at a dairy plant in Lazio Region, further investigations have been conducted both by the competent Authority and the food business operatordairy factory (as a part of dairy factory HACCP control). In total, 90 dairy products, 7 brine and 64 environmental samples have been tested. The prevalence of Listeria monocytogenes was 24.4% in mozzarella cheese, and 9.4% in environmental samples, while brines were all negatives. Forty-seven strains of L. monocytogenes have been isolated, all belonging to 4b/4e serotype. In 12 of these, the macrorestriction profile has been determined by means of pulsed field gel electrophoresis. The profiles obtained with AscI enzyme showed a 100% similarity while those obtained with ApaI a 96.78% similarity. These characteristics of the isolated strains jointly with the production process of mozzarella cheese has allowed to hypothesise an environmental contamination.
  • 关键词:Listeria monocytogenes; Mozzarella; PFGE; Serotypes
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