摘要：The authors examined the sensitivity, specificity and the detection rate of Norovirus detection from fecal specimens of the food-handlers by using Bioluminescent Enzyme Immunoassay (BLEIA) system and conventional genetic detection method (RT-PCR) by Shimadzu, and evaluated the effectiveness of the BLEIA in comparing with RT-PCR assay. A total of 176 of the 2,427 (7.3%) specimens were positive for Norovirus by the BLEIA, and 153 (6.3%) specimens were positive for Norovirus by RT-PCR. The relative sensitivity, specificity, and correspondence rate of the BLEIA for RT-PCR were 95.4, 98.7, and 98.4%, respectively. These results indicated that the BLEIA has high performance equivalent to RT-PCR. The BLEIA system could rapidly analyze 1 specimen within 46 min, and has high processing capability of 120 tests per hour. The BLEIA is considered to be useful for regular screening for Norovirus detection among a large number of fecal samples from food-handlers.
