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  • 标题:Survey of Solasodine-Type Glycoalkaloids by Western Blotting and ELISA Using Anti-solamargine Monoclonal Antibody
  • 本地全文:下载
  • 作者:Waraporn PUTALUN ; Hiroyuki TANAKA ; Shoji YAHARA
  • 期刊名称:Biological and Pharmaceutical Bulletin
  • 印刷版ISSN:0918-6158
  • 电子版ISSN:1347-5215
  • 出版年度:2000
  • 卷号:23
  • 期号:1
  • 页码:72-75
  • DOI:10.1248/bpb.23.72
  • 出版社:The Pharmaceutical Society of Japan
  • 摘要:Solasodine glycosides were analyzed for the selection of higher-producing natural resources in the fruits of Solanum spp. by western blotting and ELISA using anti-solamargine monoclonal antibody (MAb). Western blotting of Solanum khasianum showed a correlation with the analysis by ELISA. The results indicated that the fruits of S. khasianum contained the highest level of solasodine glycosides, compared to other species having 53.43±3.90 μg/mg dry wt. The high level of solasodine glycosides in S. khasianum suggests that it may be suitable for commercial production.
  • 关键词:solasodine glycoside;Solanum spp.;monoclonal antibody;western blotting;ELISA
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