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  • 标题:Action Properties of HYI Killer Toxin from Williopsis saturnus var. saturnus, and Antibiotics, Aculeacin A and Papulacandin B
  • 本地全文:下载
  • 作者:Tadazumi KOMIYAMA ; Tarou SHIRAI ; Tatsuo OHTA
  • 期刊名称:Biological and Pharmaceutical Bulletin
  • 印刷版ISSN:0918-6158
  • 电子版ISSN:1347-5215
  • 出版年度:1998
  • 卷号:21
  • 期号:10
  • 页码:1013-1019
  • DOI:10.1248/bpb.21.1013
  • 出版社:The Pharmaceutical Society of Japan
  • 摘要:The mechanism of the killing and cytocidal effects produced by HYI toxin from Williopsis saturnus var. saturnus, and by amphiphilic antibiotics aculeacin A and papulacandin B on yeast Saccharomyces bayanus cells was studied. When the yeast cells were treated with these molecules, a discharge of cell materials at the budding position was observed by phase-contrast and scanning electron microscopy. The cytocidal effect of these molecules was most pronounced when the cells were in the logarithmic growth phase. Washing the HYI toxin incubation mixture completely eliminated the killing activity, but in the case of the antibiotics, it only partially reduced the cytocidal activity. Full recovery of the killing activity in the supernatant of the washing solution was observed after XYI toxin incubation, but in the case of the antibiotics, the recovery of cytocidal activity was time-dependent. The activity of membrane β-1, 3-glucan synthase was potently inhibited by HXI toxin, and the concentration of this enzyme in the budding tip was observed. These results suggest that HYI toxin exerts a cytocidal effect on the budding of sensitive yeast cells by inhibiting cell wall synthesis. This mechanism is similar to that of the HM-1 toxin of W. saturnus var. mrakii, and to aculeacin A and papulacandin B, although there are some differences from that of HYI toxin.
  • 关键词:killer toxin;antifungal drug;β-glucan synthase;budding;cell wall;yeast
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