摘要：Glial cells are able to support neurons physically and functionally. The present study was undertaken to determine the effects of Kampo medicines on glial cell function, especially Ca<2+> mobilization. C6 rat glioma cells expressed H1-histamine, muscarinic cholinergic and adrenergic α1-receptors, simulation of which resulted in phosphoinositive hydrolysis and increase in intracellular Ca<2+> concentration ([Ca<2+>]i).The water extracts of Sho-saiko-to and San'o-shashin-to, Kampo medicines which contain Scutellariae Radix (Ogon, the root of Scutellaria baicalensis GEORI), inhibited histamine (100μM)-induced increase in [Ca<2+>]i in a concentration-dependent manner. The water extract of Scutellariae Radix potently decreased [Ca<2+>]i in a concentration-dependent manner. Sho-saiko-to, San'o-shashin-to and Scutellariae Radix significantly inhibited histamine-induced accumulation of total [3H]inositol phosphates, consistent with their inbition of the increase in [Ca<2+>]i. These results suggest that Sho-saiko-to, San'o-shashin-to and Scutellariae Radix inhibit Ca<2+> mobilization mediated via an inhibition of phospholipase C. The inhibitory effect may be important in interpreting the pharmacological actions of above Kampo medicines.
