标题：The Structure-Activity Relationship between Synthetic Butylidenephthalide Derivatives Regarding the Competence and Progression of Inhibition in Primary Cultures Proliferation of Mouse Aorta Smooth Muscle Cells
摘要：The inhibitory effects of synthetic butylidenephthalide (BP) derivatives on 10% fetal bovine serum-stimulated proliferation were assayed by measuring the proliferative cell number at an interval of 12h in primary cultures of mouse aorta smooth muscle cells (SMC). Their potencies for the anti-proliferation effect were in the order BP-42 (4, 5-dihydroxy BP)>BP-92 (4, 5-dihydroxy butylphthalide)>BP-97 (6, 7-dihydroxy-3-(3-bromo-1-octenyl)-phthalide)>BP-82 (6, 7-dihydroxy BP)>BP-86 (5, 6-dihydroxy BP)>BP-87 (4, 5, 6-trihydroxy BP)>BP-85 (4, 7-dihydroxy BP)>BP-84 (5, 7-dihydroxy BP)>BP-4C3 (4-methoxy propylphthalide)>BP-7 (4-hydroxy BP)>BP-40 (4, 5-dimethoxy butylphthalide)>BP-5C3 (4-hydroxy propylphthalide). We divided these anti-proliferative effects into anti-competence and anti-progression effects by using a convenient assay. BP-42 had the greatest potency in used phthalides for competence inhibition of the SMC proliferation. BP-92 had small potency for competence inhibition. BP-97 had greater potency for competence inhibition than BP-82. These results demonstrated that the anti-proliferative effect of BP-42 was greatest in used phthalides in primary cultures of vascular SMC. The 4, 5-dihydroxy group and 3-butylidene or 3-(3-bromo-1-octenyl) group in these synthetic BP derivatives contributed to the anti-competence effect on SMC. BP-42 may become a prototype of an anti-atherosclerotic drug.
