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  • 标题:Western Blotting for Ginseng Saponins, Ginsenosides Using Anti-ginsenoside Rb1 Monoclonal Antibody
  • 本地全文:下载
  • 作者:Noriko FUKUDA ; Hiroyuki TANAKA ; Yukihiro SHOYAMA
  • 期刊名称:Biological and Pharmaceutical Bulletin
  • 印刷版ISSN:0918-6158
  • 电子版ISSN:1347-5215
  • 出版年度:1999
  • 卷号:22
  • 期号:2
  • 页码:219-220
  • DOI:10.1248/bpb.22.219
  • 出版社:The Pharmaceutical Society of Japan
  • 摘要:Ginsenosides saparated by silica gel TLC were blotted to a polyvinylidene difluoride (PVDF) membrane which was treated with a NaIO4 solution followed by bovine serum albumine (BSA), resulting in a ginsenoside-BSA conjugate on a PVDF membrane. The blotted bands were stained with monoclonal antibody (MAb). The newly established Western blotting method was used for the determination of ginsenosides and their distribution in various Panax species.
  • 关键词:monoclonal antibody;ginseng;ginsenoside Rb1;Western blotting
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