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  • 标题:Properties and Tissue Distribution of Mouse Monomeric Carbonyl Reductase
  • 本地全文:下载
  • 作者:Shuhei ISHIKURA ; Yutaka YAMAMOTO ; Kazuya MATSUURA
  • 期刊名称:Biological and Pharmaceutical Bulletin
  • 印刷版ISSN:0918-6158
  • 电子版ISSN:1347-5215
  • 出版年度:1998
  • 卷号:21
  • 期号:8
  • 页码:879-881
  • DOI:10.1248/bpb.21.879
  • 出版社:The Pharmaceutical Society of Japan
  • 摘要:We previously cloned a cDNA for mouse cerebellum carbonyl reductase which shows more than 81% homology to the cDNAs for monomeric carbonyl reductases of the rat, rabbit and human, and for pig 20β-hydroxy-steroid dehydrogenase. In the present study, we expressed the recombinant monomeric enzyme (34 kDa and pI 8.3) from the cDNA and compared its properties with the recombinant human enzyme. The mouse and human enzymes showed similar functional properties, although they differed in kinetic constants for carbonyl substrates and in inhibitor sensitivity. Both enzymes lacked glutathione S-transferase activity. Western blot and reversetranscription-polymerase chain reaction analyses showed that the enzyme protein and its mRNA are expressed in various mouse tissues.
  • 关键词:carbonyl reductase;mRNA expression;short-chain dehydrogenase/reductase family;tissue distribution
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