摘要：We examined in vitro cytotoxic activity of imidazolyl-1, 3, 5-triazine derivatives using human breast cancer cell lines (MCF-7, R-27, T-47D and ZR-75-1) and murine leukemia cell line (P388). The percentage of viable cells was determined by the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazorium bromide (MTT) assay. Hexamethyl-melamine (HMM), a 1, 3, 5-triazine derivative has previously been recognized as an antitumor agent effective against lung, ovarian and breast cancer, but failed to show a significant cytotoxic activity in the present study. In contrast, four imidazolyl-1, 3, 5-triazine derivatives, 2-(1-imidazolyl)-4, 6-bis(morpholino)-1, 3, 5-triazine, 2-(1-imidazolyl)-4-morpholino-6-(3-thiazolidinyl)-1, 3, 5-triazine, 2-(4-cyano-4-phenylpiperidino)-4-(imidazolyl)-6-morpholino-1, 3, 5-triazine and 2-(1-imidazolyl)-4-(1-N-methyl-N-phenylamino)-6-morpholino-1, 3, 5-triazine showed cytotoxic activity for most cell lines, which was significantly greater than the activity of hydroxymethylpentamethylmelamine (HMPMM), a major metabolite of HMM.
