标题：A Novel Enzyme-Linked Immunosorbent Assay for Quantification of Soybean β-Conglycinin, a Major Soybean Storage Protein, in Soybean and Soybean Food Products
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：2005
卷号：51
期号：1
页码：34-39
DOI：10.3177/jnsv.51.34
出版社：Center for Academic Publications Japan
摘要：Soybean ( Glycine max L.) storage proteins are composed of two major compo-nents, β-conglycinin and glycinin, corresponding to 75 and 11S globulins, respectively. Recently, soybean β-conglycinin (7S globulin) has been reported to show beneficial func-tions in animals and human. To date, there is no method for the precise quantification of soybean β-conglycinin in processed food products or soybean seeds. We report here a novel method for this purpose. At first, antibodies specifically reactive to the subunits of β-congly-cinin were prepared. And then, a direct enzyme-linked immunosorbent assay (ELISA) for the quantification of soybean, β-conglycinin in processed foods and seeds was developed. In this assay, the sample was treated with sodium dodecyl sulfate sample buffer followed by dilution with phosphate-buffered saline. The diluted samples were poured and coated onto an ELISA plate and reacted with rabbit anti-β-conglycinin antibody and peroxidase-labeled anti-rabbit IgG. Finally, the bound peroxidase-labeled antibody was detected by colorimetric reaction. By using this system, it has been possible to measure soybean β-conglycinin con-centrations in several processed food products. In addition, this simple quantification ELISA system was demonstrated to be adaptable for the quantification of β-conglycinin contents of various soybean cultivars.
