期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：2001
卷号：47
期号：1
页码：69-77
DOI：10.3177/jnsv.47.69
出版社：Center for Academic Publications Japan
摘要：Arachidonic acid (20:4 n -6) and its metabolic products, such as prosta-glandins and leukotrienes, have been known to be associated with skin inflammatory reac-tions. However, the mechanism of the competitive incorporation of 20:4 n -6 into ke-ratinocytes among polyunsaturated fatty acids (PUFAs) remains uncertain. To investigate the relationship between the molecular structure of PUFAs and the rate of incorporation of PUFAs into cells, a fetal rat skin keratinocyte (FRSK) cell line was used. The cells were incu-bated for 24 h with any two of the following arachidonic acid analogs: mead acid (20:3 n -9), dihomo-γ-linolenic acid (20:3 n -6), 11, 14, 17-cis-eicosatrienoic acid (20:3 n -3), arachi-donic acid (20:4 n -6), eicosapentaenoic acid (20:5 n -3) and 5, 8, 11, 14-cis-nonadecate-traenoic acid (19:4 n -5), at the ratio of 1:0, 0.5:0.5, or 0:1; and their incorporation into lipid was measured by capillary gas-liquid chromatography. The experiments indicated that 20:3 n -6 was preferentially incorporated into phospholipids of FRSK rather than 20:3 n -9 or 20:3 n -3, and 19:4 n -5 as well as 20:4 n -6 was preferentially incorporated into total cel-lular lipid and phospholipids rather than 20:3 n -9 or 20:5 n -3. When two PUFAs were added simultaneously to the medium, 19:4 n -5 most effectively reduced the competitive i n -corporation of 20:4 n -6 into phospholipids. These results suggest that keratinocytes dis-criminate 20:4 n -6 from other arachidonic acid analogs by its double bond positions from the carboxyl group.
