期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：2000
卷号：46
期号：6
页码：321-324
DOI：10.3177/jnsv.46.321
出版社：Center for Academic Publications Japan
摘要：A simple and rapid analysis of total ascorbic acid (AsA) in serum and plasma and its automated analysis are described. AsA is oxidized by ascorbate oxidase (AsA oxi-dase) to dehydroascorbic acid that then reacts with o-phenylenediamine (OPDA) to form a quinoxaline derivative that absorbs at 340 nm. The change in absorbance is directly propor-tional to the total AsA concentration. The assay was validated with a linear concentration range of 0.8-80mg/L, and the within-day and between-day assays precision did not exceed 8.6% and 12.5%, respectively. On 47 sera, the manual enzymatic procedure gave 0.2mg/L on average lower values than those of an automated enzymatic procedure with a correla-tion coefficient of 0.847. On another 66 sera, results by automated enzymatic method cor-related well with the HPLC method and the regression equation is Y (enzymatic, automated)=0.97 X (HPLC)+0.1, r =0.980, Sy.x=0.6mg/L. An experienced analyst can perform about 24 manual assays per hour whereas the automated procedure gave a rate of 100 assays per hour.<
关键词：vitamin C determination;ascorbic acid;ascorbate oxidase;automated assay;enzymatic methods
