期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：2000
卷号：46
期号：5
页码：215-221
DOI：10.3177/jnsv.46.215
出版社：Center for Academic Publications Japan
摘要：A method for the sensitive and specific determination of folate derivatives was developed. The method involves hydrolysis by γ-glutamyl hydrolase and high-performance liquid chromatography with electrochemical detection. The method was applied to measure the change in the level of folate derivatives in the liver, kidney, spleen and brain of rats during folate deficiency. 5, 6, 7, 8-Tetrahydrofolic acid was the major folate derivative in the liver, kidney, spleen and brain. Total concentration of folate derivatives decreased from the second week of folate deficiency in the liver, kidney, spleen and brain followed by anemia, which ap-peared at the fifth week. The level of 5, 6, 7, 8-tetrahydrofolic acid in the brain did not change during folate deficiency, but it significantly decreased in the liver, kidney and spleen.
