期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：2000
卷号：46
期号：4
页码：175-179
DOI：10.3177/jnsv.46.175
出版社：Center for Academic Publications Japan
摘要：The effects of nucleotide and nucleoside supplementation on the formation of tight junctions and the expression of microvilli, as indexes of morphological differentiation were studied by using a human colon adenocarcinoma cell line (Caco-2 cells). The forma-tion of tight junctions and the expression of microvilli were evaluated by measuring the transepithelial electrical resistance (TEER) and observing the cell surface under electron mi-croscopic analysis, respectively. To clarify the nutritional significance of human milk nu-cleotides, we used a nucleotide mixture (and a corresponding nucleoside mixture) with a composition similar to that found in human milk. Nucleotides had no effect on TEER, but nucleosides markedly promoted the increase of TEER. When alkaline phosphatase activity in the brush border membrane was enhanced by the addition of triiodothyronine (TIT), nu-cleotides also promoted the increase of TEER. Cytidine and CMP predominant in the mix-ture influenced the increase of TEER materially. Furthermore, an electron microphotograph of the cell surface showed that nucleosides contributed to the expression of microvilli. Thus the results presented in this study suggest that nucleotide and nucleoside supplementation may enhance the morphological differentiation of Caco-2 cells.
