期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：2000
卷号：46
期号：2
页码：97-100
DOI：10.3177/jnsv.46.97
出版社：Center for Academic Publications Japan
摘要：Forms of vitamin B12 (B12) compounds in young and aged corbiculas were ex-amined by bioautography using B12-requiring microorganisms combined with cellulose ac-etate membrane electrophoresis or high-performane liquid chromatography (HPLC). Both corbiculas (per 100 g) contained ca. 30 ug of cobalamin, a complete type of B12. Five known B12 compounds, adenosylB12(AdoB12), methylB12, hydroxoB12, sulfitoB12 and cyanoB12, were identified by bioautography. Young corbicula contained more B12 compounds, as-sumed as methylB12, AdoB12 and sulfitoB12, as compared to aged corbicula. All of the B12 compounds detected around the location corresponding to those of methylB12, AdoB12 and sulfitoB12 in the bioautography were converted into compounds that behaved like hydroxoB12 after photolysis. Young corbicula was found to contain an unidentified B12 com-pound using bioautography combined with HPLC. A large portion of the B12 compound that moved like hydroxoB12 during cellulose acetate membrane electrophoresis using 0.5 N acetic acid might be identical with the unidentified B12 compound detected in the HPLC-bioautog-raphy.
关键词：vitamin B12;forms of vitamin B12;sulfitoB12;corbicula
