标题：Vitamin E Suppresses the Induction of Reactive Oxygen Species Release by Lipopolysaccharide, Interleukin-1β and Tumor Necrosis Factor-α in Rat Alveolar Macrophages
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1999
卷号：45
期号：6
页码：675-686
DOI：10.3177/jnsv.45.675
出版社：Center for Academic Publications Japan
摘要：Over the last decade, although investigations have suggested that vitamin E affects the immune response, not much is known about its affect on the alveolar macrophage functions. In the present study, we have investigated the effect of high vitamin E (DL-α-tocopheryl acetate, α-TA) supplementation for 10d on the activation state of rat alveolar macrophages induced by lipopolysaccharide (LPS), interleukin (IL)-1β or tumor necrosis factor (TNF)-α on the basis of their ability to produce reactive oxygen species (ROS), such as superoxide (O-2) and H2O2. LPS treatment (1 and 10μg/mL) caused 2.44 and 2.54-fold increases in O-2, and 2.1 and 2.3-fold increases in H2O2, respectively, from alveolar macrophages (AMs) in the diet group fed 50mg α-TA/kg. However, this enhancement was not observed for the AMs of the diet groups fed 250 or 1, 250mg α-TA /kg. Similar results were obtained on treating the AMs with proinflammatory cytokines IL-1β or TNF-α. The observed suppression in ROS release in response to various stimulants may be due to the direct and/or indirect effect of high vitamin E (250 and 1, 250mg α-TA/kg diet) supplementation. It may therefore, be concluded that high α-TA supplementation in the diet modulates the activation of AMs in rats.
