期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1977
卷号：23
期号：2
页码：133-143
DOI：10.3177/jnsv.23.133
出版社：Center for Academic Publications Japan
摘要：The trypsin inhibitor in eggplant, Solanum melongena L., was isolated and purified by the improved method with the techniques of dialysis using acetylated cellulose tube and ion-exchange chromato-graphy on DEAE-Sephadex. The final preparation was found to be homogeneous by disc and SDS-polyacrylamide gel electrophoreses. This inhibitor had the molecular weight of about 6, 200, the pI value of 4.7, and furthermore characteristic amino acid composition lacking in trypto-phan, histidine, valine and methionine. The trypsin inhibition data indicated that the purified inhibitor combined with bovine trypsin [EC 3.4.21.4] in the molar ratio of 1:1. These properties of this inhibitor were in agreement with those of the dialyzable eggplant trypsin inhibitor pre-viously purified, indicating that the dialyzable and non-dialyzable inhi-bitors in eggplant are identical.
