标题：FURTHER CHARACTERIZATION OF RENNIN ACTION ON κ-CASEIN USING CARBOXYMETHYLCELLULOSE: EFFECTS OF VARIOUS ADDITIVES ON THE ENZYMATIC HYDROLYSIS OF κ-CASEIN
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1977
卷号：23
期号：2
页码：153-159
DOI：10.3177/jnsv.23.153
出版社：Center for Academic Publications Japan
摘要：The effects of various additives on the reaction of rennin with κ-casein were investigated by using carboxymethylcellulose. Both urea and sodium 1-anilino-8-naphthalenesulfonate effectively inhibited rennin action at concentrations larger than 2M and 2mM, respectively. These reagents, however, activated the enzyme action at the lower concentrations. Both αs- and β-caseins had some ranges of concen-trations in which the rennin reaction was activated. Calcium chloride had an inhibitory effect on the rennin action. Neither mercaptoethanol nor KCl had any appreciable effect on the enzymatic hydrolysis of β-casein. These results are analyzed in terms of the association and dissociation of β-casein due to the presence of these additives in the reaction solutions.
