摘要：A gas-liquid chromatographic (GLC) method was developed for the determination of diethylstilbestrol glucuronide (DESG), a metabolite of diethylstilbestrol, in beef. DESG was extracted from beef with 90% acetonitrile. The extract was cleaned up without hydrolysis of DESG by Sep-pak NH2 cartridge and C18 cartridge. The purified extract was evaporated to dryness and reacted with pentafluoropropionic acid anhydride (PFPA) for 50 min at 50°C. PFPA derivatives of DESG were measured by GLC, using a 2% OV-17 column and electron capture detector. Recoveries of DESG obtained from beef spiked at 0.005-0.02 μg/g (as is DES) levels were 86.8-89.2%. The detection limit of DESG was 0.001 μg/g.
