出版社：National Institute of Occupational Safety and Health
摘要：Mutagenicity of the urine extracts of the rats to which either o -tolidine or Evans Blue, an o -tolidine-based azo dye, had been orally given was assayed in the Salmonella/mammalian-microsome test. The urine extracts obtained from the rats treated with o -tolidine showed stronger mutagenicity than o -tolidine itself. While Evans Blue did not show mutagenicity at all, the urine extracts derived from the substance were found mutagenic. Both 3, 3'-dimethyl- N -acetylbenzidine and 3, 3'-dimethyl- N , N '-diacetylbenzidine were identified as urinary metabolites of o -tolidine. They were found not only in the urine of the rats treated with o -tolidine but also in the urine of the rats to which Evans Blue had been orally administered. The experimental results suggest that the accidental intake of biphenyl-based dyestuffs through the mouth should be cautiously avoided. Some aspects of the metabolism of o -tolidine and Evans Blue are discussed along with the problem associated with the mutagenic activities of the o -tolidine metabolites.
